The Varioskan LUX multimode reader has received HTRF™ certification from Cisbio Bioassays and DLReady™ validation from Promega Corporation. Unlike fluorescence intensity which focuses on the quantification of emission intensity at a specific wavelength and neglects its polarization, fluorescence polarization specifically analyses as output the emission intensity of. + 4 µL HTRF ® pre-mixed antibodies . The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. Dual monochromators for assay flexibility With. MUTANT HTT STANDARD WORKING SOLUTIONSLysis buffer 4X for two-plate assay protocol on suspension cells Determine the amount of lysis buffer needed for the experiment. Read & analyse. Make sure the setup for Tb Cryptate is used. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. FOR HTRF CERTIFIED READER. PURCHASE SEPARATELY . They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. overnight at 4 C [Prostaglandin E2] pg/ml. The Infinite 200 PRO is an easy-to-use multimode plate reader family that offers affordable, high performance detection solutions, referenced in more than 1800 peer reviewed publications. The plate map included a low and a high level calibrator, conjugate blanks, and a specific control to assess the counting rate at 620 nm (i. These plates can be read on all HTRF certified readers, thus making their implementation in. You can verify your reader’s capacity to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. 1) . Read & analyse. . no. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Lysates are then transferred to the assay plate for the detection of total cRaf by HTRF. An. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. Lyse cells Read on an HTRF compatible reader . The reader only allows high performance for the used HTRF measurement when the HTRF is run with white plates. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Small ONE- PLATE ASSAY PROTOCOL: overnight FOR HTRF CERTIFIED READER . Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Both readers demonstrated their ability to detect IP1 concentrations according to Cisbio’s acceptance criteria. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. Plate 25 μL of cells in. Set up your reader for Eu3+ Cryptate and read the fluorescence emission at two different wavelengths (665nm and 620nm) on a compatible HTRF® reader**. For more information about HTRF. Read other about this measurement approach here. If not, our scientific team will help you choose the instrument that best suits your needs and budget. Dual-Luciferase® (DLR™) gene assays. They can be used in a no-transfer. FOR HTRF CERTIFIED READER. Seal the plate and. PerkinElmer EnVision Multimode Plate Reader. Plus . Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. The optical systems use two scanningHighly compatible plates for HTRF. Specialized filters allow for IR fluorescence. For the standard curve described, all wells of the microplate are read. HTRF Microplate Readers. For more information about HTRF ®The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Step 1 Step 2 Step 3 Step 4 Step 5 Step 6. Lysates are then transferred to the assay plate for the detection of Total-eNOS by HTRF. Your lab might even have one already. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection The SpectraMax M5e Reader offers the additional benefit of being certified for Cisbio Bioassays’ HTRF® technology. The new ligands are divided into four groups according to their chemical structures and/or interactions with YTHDC1 (Table 1 and Fig. • Select Excitation Filter 320x40 HTRF Eu cryptate* • Select Excitation Optic Empty • Select Emission Filter 495xm10uv • Select Emission Optic 2 – FPThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. For more information about HTRF ®Excitation range. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance forThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Read & analyse. 14 seconds for a 384-well plate. The HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. 47 seconds for a 1536-well plate. For more information about microplate recommendations, please visit our website • Store the kit at -16°C. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. These plates can be read on all HTRF certified readers, thus making their implementation in. PerkinElmer offers two microplate readers certified for use with HTRF: Envision. SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3 Ordering information VWR cat. Both readers demonstrated their ability to perform the cAMP G s HiRange assay with results well within Cisbio’s required. Lysates are then transferred to the assay plate for the detection of total-Tau by HTRF. MATERIALS PROVIDED: KIT . SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Plus . 96-well culture plateFOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED READER For more information about HTRF. 5 µL. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. (665 nm and 620 nm) on a compatible HTRF ® reader. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. High Speed Monochromator offers a typical wavelength accuracy of <0. • HTRF®-Certified Reader. The EnSight<sup>®</sup> multimode plate reader offers HTS Alpha and Ultra-sensitive Luminescence technologies alongside conventional multimode detection technologies. Remove the plate seal and read on an HTRF compatible reader. + 4 µL HTRF pre-mixed antibodies . The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Incubate at room temperature. Typical applications The HTRF cartridge provides optimal performance for key applications. Table 1. The detection antibody is incubated for 1. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. Part Number# Product Description Number of Plates# 66PL96001 HTRF 96 well low volume plate 1 plate 66PL96005 HTRF 96 well low volume plate 5 platesThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. White 384-well low volume plate Make sure to use the set-up for Eu3+ Cryptate. The Negative control is used to check the non-specific signal. Lysates are then transferred to the assay plate for the detection of total-Tau by HTRF. 5. Lysates are then transferred to ® the assay plate for the detection of Total EGFR L858R by HTRF reagents. Setu CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified. Lysates are then transferred to the assay plate for the detection of total-STING by HTRF. The tried, tested, and proven EnVision plate reader has an array of exciting features that can enhance your applications. Plate Type and Read Area should be selected based on the microplate used and wells to be read. The perfect microplate reader for everyday biochemical and cell-based assays, it measures at any wavelength, with top and bottom reading of all technologies. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Microplate Readers and Reader Accessories. But sometimes these parameters are not sufficient enough. FOR HTRF CERTIFIED READERThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Make sure the setup for Eu Cryptate is used. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. 96-well or 384-well small volume (SV) detection microplates - For more information about microplate recommendations, please. Lysates are then transferred to the assay plate for the detection of total-CREB by HTRF. • HTRF®-Certified Reader. HTRF is compatible with a large range of available microplate readers. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. At Cisbio we provide microplates that help you discover better. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. The SpectraMax® M5e includes htrf certification, and both the M5 and M5e are lanthascreen certified. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERHTRF® 96-well low volume plate TECHNOLOGY ALL THE BENEFITS OF HTRF WITH THE EASE OF USE OF AN ELISA PLATE!. 1 – 1. 1. Make sure the setup for Tb Cryptate is used. After an overnight incubation, the plate is washed 3 times. If not, our scientific team will help you choose the instrument that best suits your needs and budget. Setu CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified. EnSight ® offers both fast well-imaging and multimode detection capabilities for target-based and phenotypic screening, all in a single benchtop instrument. They can be used in a no-transfer protocol with suspension cells or with. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. Read & analyse. Customer Transformation Hub. FOR HTRF CERTIFIED READER. These plates can be read on all HTRF certified readers, thus making their implementation in. It continues to deliver optimal results. FOR HTRF CERTIFIED READER. The donor and acceptor can be used to label a variety of biomolecules, for. For more information about HTRF. • HTRF®-Certified Reader **. Fluorescence intensity – Fluorescence intensity is one of the most popular detection modes, and there are innumerable fluorophores available for applications such as DNA or protein quantitation, reporter-geneCertified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. For more information. Flexibility combined with the best sensitivity of its class, and our newly developed Enhanced Dynamic Range technology, make it the ideal reader for assay development. Incubate overnight at RT. For more information about HTRF. Choosing the right microplate reader ensures you’ll get an optimal readout. For more information about HTRF ®The LUMIstar® Omega is BMG LABTECH's powerful dedicated luminescence microplate reader, providing highly sensitive flash and glow assay readings. The plate reader. Lysates are then transferred to the assay plate for the detection of Total Cofilin by HTRF® reagents. Reagents were dispensed following Cisbio’s ligand binding protocol in 384-well format (white plates). FOR HTRF CERTIFIED READER. For more information about HTRF ®• HTRF®-Certified Reader. • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readers HTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. In particular, these parameters should be entered as defined in the table below Setup Top filter cube EX 330 / LUM (block #18) EM 620 / 665 / LUM (block #41) Light source LaserDetect flash, glow, Dual Luciferase ® Reporter and BRET assays. 10 µL Standard or Sample. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. FOR HTRF CERTIFIED. FOR HTRF CERTIFIED READER For. HTRF TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: Add ONE-PLATE ASSAY PROTOCOL: overnight * * Depending on cell lines used, volume of lysis should be optimized. 2400 for 665 and 620 with white plate HTRF® Europium cryptate donor / Red acceptor readout. It also integrates with the. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option includes. Time-resolved fluorescence was measured switch the SpectraMax i3x and SpectraMax iD5 readers using preconfigured protocols in SoftMax® Pro Software (see Tables 2 and 3 for instrument-specific settings). It can also be further streamlined to a one-. Make sure the setup for Tb Cryptate is used. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. -Certified Reader. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™- certified. The HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. HTRF is a versatile TR-FRET technology developed by Cisbio for detecting biomolecular interactions. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. No washing steps are required. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within theassay linear range 6 Add 4 μL of premixed antibody solutions (vol/vol) prepared in the detection buffer. Remove the plate seal and read on an HTRF compatible reader. 96-well culture plateHTRF certified . Microplate reader assays requiring infrared (IR) or. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Lysates are then transferred to the assay plate for the detection of phospho-c-Src (Tyr419) by HTRF. It also integrates with the powerful SoftMax Pro Software for. FOR HTRF CERTIFIED READER. TO PREPARE TISSUE SAMPLES: Please refer to the Technical Note “Optimize Your HTRF® Cell Signaling Assays on Tissues” onHow to use Synergy microplate readers for HTRF detection, a versatile technology for drug discovery and life science research. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. * For HTRF microplate recommendations, please visit . microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. Read & analyse. FOR HTRF CERTIFIED. HTRF is a proprietary time-resolved fluoresce nce technology that overcomesCertified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. , filters and/or monochromators), a second optical system for the selection of emission light, and a detector, usually one or multiple photomultiplier tubes (PMTs). As shown in Fig. • HTRF cAMP HiRange Assay Kit (Cisbio Bioassays, France) • 96-well half area plates (white) (Greiner Bio-One, Germany) • 384-well small volume plates (white and black) (Greiner Bio-One, Germany) HTRF RCK The HTRF RCK is used to confirm the general suitability of a reader for HTRF assays, as well as set up and performance validation. • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readersHTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Make sure the setup for Tb3+ Cryptate is used. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. 1. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. FOR HTRF CERTIFIED READER . Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Plate 25 μL of cells in. + 4 µL HTRF ® pre-mixed antibodies . Microplate readers are only certified if they. Catalog No. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: + 10 µL lysis buffer ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Because of its homogeneous and fast nature, the assay is a preferred method to determine the protein concentration of samples. ONE- PLATE ASSAY PROTOCOL: 5 µL cells + 5 µL compounds (or stimulation buffer) + 5 µL agonist + 5 µL HTRF ® pre-mixed antibodies * Depending on cell lines used, volume of lysis should be optimized. + 4 µL HTRF pre-mixed antibodies . Specialized microplate readers are available that enable HTRF (Homogeneous Time-Resolved Fluorescence) experiments and analyses. Thanks to HTRF signal stability, the assay plate may be used to check several instruments, or. Discover our high performance reader. For more ® information about HTRF compatible readers and for set-up recommendations. The SpectraMax ® iD5 Multi-Mode Microplate Reader is HTRF-certified, with an enhanced TRF detection module and filters optimized for HTRF detection. These plates can be read on all HTRF certified readers, thus making their implementation in. For more information about HTRF. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. FOR HTRF CERTIFIED. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. FOR READING, AN HTRF ®-CERTIFIED READER IS NEEDED. It measures 96-well plates in less than 20 seconds and 1536-well formats in under one minute. The ratio between control lysate signal / non-specific signal should be greater than 2. Make sure the setup for Eu3+ Cryptate is used. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. For a list of HTRF-compatible readers and set-up recommendations, please visit . The assay was prepared in both white and black plates and incubated >18 hours. FOR HTRF CERTIFIED READER. Plate 1 plate HTRF 96-well low volume plate *To prepare working standard solutions, culture medium can be an alternative the diluent. + 4 µL HTRF ® pre-mixed antibo dies 2. Choose from over 50 certified or compatible plate readers More than 50 certified or compatible instruments, and counting! Your lab might even already have one. Incubate 4h at RT. Certified HTRF compatible reader by CisBio Bioassays;Here’s how it works: we come to you with over 30 years of plate reader instrument development experience. FOR HTRF CERTIFIED READER. 8 µL cells. FOR HTRF CERTIFIED READER. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. support such assays and even feature endorsements from reagent suppliers saying the reader is “HTRF compatible,” “LanthaScreen Certified Plus,” Transcreener Validated” or. For more. For more information about microplate recommendations, please visit our website STORAGE AND STABILITYONE-PLATE ASSAY PROTOCOL: Small volume detection plate. This DLReady-certified microplate reader can also come equipped with Simultaneous Dual Emission for BRET applications and can be further upgraded to a multi-mode reader. Make sure the plate reader is turnedon and then open the MikroWin software on the computer. An HTRF assay measures the fluorescence energy transfer between molecules in close proximity. Learn about the principles, applications, and benefits of HTRF in this Agilent application note. 96-well plate: 0 to 4 Abs, ±2% at 450 nm, 384-well plate: 0 to 3 Abs ±2% at 450 nm:. </p> <p>And, the Enhanced Security software option includes tools to facilitate 21 CFR Part 11 compliance for integration into regulated environments (GxP). spectramax paradigm system, htrf reagents, spectramax i3 and paradigm readers, HTRF assay, htrf certifiedLysates are then transferred ®to the assay plate for the detection of Total CHK2 by HTRF reagents. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies Read & analyse Incubate overnight at RT Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. For more. Application Area: absorbance, fluorescence, luminescence, and homogeneous time resolved fluorescence assay development. Make sure to use the set-up for Eu Cryptate. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. For multiplexed luminescent and fluorescent assays, using a white plate will support maximum light output signal for the luminescent portion of the assay, but result in higher crosstalk and background for the fluorescent portion of the assay. Optimized instrument settings for the HTRF camps HiRange assay. microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. Other technologies such as imaging. Microplate readerFluorescence microplate readers usually contain a light source, an optical system for the selection of excitation light (i. Transcreener® ADP2 TR-FRET assay. HTRF (Homogeneous Time Resolved Fluorescence) is a no-wash technology. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Read & analyse. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. It is typically used to determine the concentration of nucleic acids and proteins. Lysates are then transferred to the assay plate for the detection of Phospho-AKT-2 (Ser473) by HTRF. acceptor reagent. Time-resolved fluorescence was measured on the SpectraMax Multi-Mode Microplate Readers (see Table 2 for instrument settings). • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readersONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Provide more sensitive and precise results. For more information about HTRF ®Highly compatible plates for HTRF. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. Read & analyse. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. The SpectraMax M5e Reader offers the additional benefit of being certified for Cisbio Bioassays’ HTRF® technology. VICTOR® Nivo™ multimode plate reader is the smallest plate reader on the market with all major detection technologies including Alpha and certification for HTRF® technology plus software designed for 21 CFR Part 11. FOR HTRF CERTIFIED READER. View Brochure. EX: HTRF Emission range. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. Plate 25 μL of cells in. It guarantees high level of cAMP accumulation in the cells. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. At Cisbio we provide microplates that help you discover better. As shown in Figure 1, the interaction between Tag1-SOS1 and Tag2-KRAS-WT is detected by using anti-Tag1-Terbium (HTRF donor) and anti-Tag2-XL665 (HTRF acceptor). 96-well culture plateFOR HTRF CERTIFIED READER For more information about HTRF. FOR HTRF CERTIFIED READER. Read & analyse. Lysis buffer 1X for assay protocol on tissues, two-plate assay protocol on adherent cells, and standard dilution. 41 mg/ml) was obtained from Thermo Fisher Scientific. The measurement of HTRF assays is predominantly performed on microplate readers. Lyse cells Read on an HTRF compatible reader . Lysates are then transferred to the assay plate for the detection of total-p53 by HTRF. • HTRF®-Certified Reader. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. (2) Forskolin activates the adenylyl cyclase enzyme and increases the intracellular level of cAMP. It guarantees high level of cAMP accumulation in the cells. It comes equipped with our patented LVF Monochromators™, filters, and spectrometer. Preconfigured HTRF protocols in SoftMax ® Pro Software 7. It combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background. Read & analyse. FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations, please visit our. Lysates are then transferred to the assay plate for the detection of total cRaf by HTRF. Revvity. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations, please visit our. FOR HTRF CERTIFIED READER. This protocol enables the cells' viability and confluence to be monitored. FOR HTRF CERTIFIED READER. HTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. 25,000 human Jurkat cells were plated in 96 well plates. For more ® information about HTRF compatible readers and for set-up recommendations, please. 55 µL anti-cAMP-Cryptate. Excitation lasers significantly improve performance and lower. This protocol, HTS designed, allows miniaturization while. The optional HTRF-certified filter-based module may be. 0. If not, our scientific team will help you choose the instrument that best suits your needs and budget. It enables you to gain insights you couldn’t achieve with detection measurements alone. FOR HTRF CERTIFIED READER. Results for HTRF Reader Control Kit with black and white microplates. . They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. For more ® information about HTRF compatible readers and for set-up recommendations, please. As shown in Fig. Make sure the setup for Tb Cryptate is used. Endpoint mode experiments measure each well of the microplate only once. White 384-well low volume plate Make sure to use the set-up for Eu 3+ Cryptate. For more information about microplate recommendations, please visit our website STORAGE AND STABILITY• HTRF®-Certified Reader. Remove the plate sealer and read on an HTRF® compatible reader . FOR ORE INFORMATION Europe and other ountries 33(0)466 96 0 U. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. The ratio of the two fluorescence TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: + 10 µL lysis buffer ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. The EnVision XCite multimode plate reader provides exceptional sensitivity across all detection technologies, while keeping you flexible with monochromator option and full upgradeability. In addition, stacker. Conclusion. Better sensitivity provides better statistic, less variability among replicates, and a larger assay window. EnVision Nexus ultimode Plate Reader 2 Benefits • Provides the speed and sensitivity to boost your lab’s productivity with ultra-high throughput • Freely configurable and upgradable to adapt to your needs today and tomorrow • Fully automatable for walkaway convenience • Certified for use with HTRF® technology Key featuresBMG LABTECH plate readers certified for LanthaScreen detection include the PHERAstar FSX, CLARIOstar Plus and VANTAstar. **For more information about HTRF® compatible readers and for set-up recommendations, please visit our website at:ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The FLUOstar ® Omega is a multi-mode microplate reader with three detection modes. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. 3nm, important when reading at 280 nm for DNA analysis, for more exact results. FOR HTRF CERTIFIED READER. Figure 1. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. View Brochure. This protocol gives the cells viability and confluence to be monitored. Both readers demonstrated their ability to detect IP1 concentrations according to Cisbio’s acceptance criteria. For more information about HTRF ® compatible readers and for set-up recommendations, please. The optimized well design and high quality plastics provides low background and crosstalk. FOR HTRF CERTIFIED READER. • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readersFOR HTRF CERTIFIED READER. The adapterResults summary for HTRF IP1 standard curve. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: 25 µL cells ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. The Z´ value takes into account four (4) parameters: the means (µ) and the standard deviations (σ) of the positive (plateau) and the negative (baseline) controls 1. 96-well culture plateThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same . Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. This protocol gives the cells viability and confluence to be monitored. Revvity.